Journal article
High-resolution complementary chemical imaging of bio-elements in Caenorhabditis elegans
DJ Hare, MWM Jones, VC Wimmer, NL Jenkins, MD De Jonge, AI Bush, G McColl
Metallomics | ROYAL SOC CHEMISTRY | Published : 2016
DOI: 10.1039/c5mt00288e
Abstract
Here, we present a sub-μm multimodal approach to image essential elements in Caenorhabditis elegans. A combination of chemical imaging technologies reveals total metal concentration, chemical state and the protein to which an element is associated. This application of distinct yet complementary chemical imaging techniques provided unique insight into essential and trace elements at the subcellular level.
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Grants
Awarded by National Institutes of Health
Funding Acknowledgements
An Australian Research Council Discovery Project (DP130100357) to A. I. B., G. M. and M. D. J. and a UTS Chancellor's Postdoctoral Fellowship to D. J. H. supported this work. We would like to thank the XFM beamline at the Australian Synchrotron, Kirsten Grant, Fransisca Sumardy and Ian Birchall (Florey) for technical assistance, the Caenorhabditis Genetics Center (CGC) supported by the US National Institutes of Health - Office of Research Infrastructure Programs (P40 OD010440) for providing strains, and the Victorian Government's Operational Infrastructure Support Program.